Acute Response Capsaicin Flare Study

• Drug: PF-04427429
  
  Single dose IV infusion up to 300mg
• Other: Capsaicin challenge
  
  Capsaicin challenge: 110 mg (0.075% capsaicin) to be applied at pre-dose and at 2h, 5h, 8h and 24h post-dose as well as 1 week and 4 weeks post final treatment period
• Drug: Placebo
  
  Placebo IV infusion (saline) to be administered during two of the three treatment periods
• Other: EMLA positive control
  
  2g EMLA cream to be applied ONCE during study period 1

• Experimental: PF-04427429
  
  Interventions:
  

  • Drug: PF-04427429
  • Other: Capsaicin challenge
• Placebo Comparator: Placebo
  
  Interventions:
  

  • Drug: Placebo
  • Other: Capsaicin challenge
• Active Comparator: EMLA
  
  Intervention: Other: EMLA positive control

Inclusion Criteria:

• Healthy male subjects between the ages of 18 and 50 years inclusive. Healthy is defined as no clinically relevant abnormalities identified by a detailed medical history, full physical examination, including blood pressure and pulse rate measurement, 12-lead ECG and clinical laboratory tests.
• Evidence of a personally signed and dated informed consent document indicating that the subject (or a legally acceptable representative) has been informed of all pertinent aspects of the trial.
• Skintype I-III with a distance from base of scaphoid to antecubital fossa >26cm on their forearms.

Exclusion Criteria:

• Evidence or history of clinically significant hematological, renal, endocrine, pulmonary, gastrointestinal, cardiovascular, genitourinary, psychiatric, neurologic, or allergic disease (including drug allergies, but excluding untreated, symptomatic, seasonal allergies at time of dosing).
• Use of biologics including any live vaccines within 3 months prior to screening or prior treatment with an antibody in a previous clinical trial or treatment with an immunoglobulin in the past 6 months.
• 12-lead ECG demonstrating QTcF >450 msec at screening.
• Subjects with scars or tattoos on the forearms, or with evidence of sunburn, or excessive hairy volar forearms.